microRNA-146a targets the L1 cell adhesion molecule and suppresses the metastatic potential of gastric cancer.
نویسندگان
چکیده
Recent studies have shown that microRNA-146a (miR-146a) is associated with cancer metastasis. However, the mechanisms underlying this process remain poorly understood. In this study, we aimed to investigate the potential role of miR-146a in gastric cancer metastasis. A wound-healing assay and a Transwell assay were used to investigate the impact of miR-146a on the migratory and invasive abilities of MKN-45 cells in vitro. MKN-45 cells stably expressing miR-146a or the negative control were transplanted into nude mice through the lateral tail vein to explore the effect of miR-146a on tumor metastasis in vivo. A luciferase reporter assay and western blot analysis were used to identify the potential target genes. Our results show that the overexpression of miR-146a inhibits the invasion and metastasis of MKN-45 cells in vitro and in vivo. Furthermore, the L1 cell adhesion molecule (L1CAM) was identified as a novel target of miR‑146a in gastric cancer. Taken together, our results provide evidence that miR-146a suppresses gastric cancer cell invasion and metastasis in vitro and in vivo, which may be in part due to the downregulation of L1CAM. miR-146a may have the therapeutic potential to suppress gastric cancer metastasis.
منابع مشابه
The effect of microRNA-125 on the adhesion molecule expression of integrin beta2 and adhesive determination of endothelial cells isolated from human aorta to monocyte
Background: The immune-mediated responses in vascular cells may include the increased expression of endothelial adhesion molecules, leukocyte rolling and infiltration, cellular lipid dysregulation and vascular smooth muscle cells (VSMCs) differentiation. Investigating the cellular and molecular events involved in the rolling process is useful for treatment or prevention of the vessel stenosis es...
متن کاملSoluble Intracellular Adhesion Molecule-1 in the Serum of Colon and Gastric Cancer Patients
متن کامل
Molecular targets of pomegranate (Punica granatum) in preventing cancer metastasis
Metastasis is the primary cause of mortality and morbidity among cancer patients and accounts for about 90% of cancer deaths. The most common types of treatment for cancer metastasis are chemotherapy and radiotherapy. However, such therapy has many serious side effects that could diminish the quality of life in patients. There is increased appreciation by the scientific community that natural c...
متن کاملMicroRNA Upregultion in MKN-45 Gastric Cancer Cell Line by Ibuprofen Treatment: Highlighting the Importance of miR-22
Nonstroidal anti-inflammatory drugs (NSAIDs) including ibuprofen have been shown to decrease the growth of cancer cells. Owning to the involvement of microRNAs in development of cancer, the aim of this study was to evaluate the effect of ibuprofen on the expression level of some microRNAs including hsa-miR-4290, hsa-miR-22 and hsa-Let-7b in MKN-45 cells. First, the growth inhibitory effect of i...
متن کاملبررسی میزان بیان microRNA-146a در پولیپ های آدنوماتوز و سرطان کولورکتال
Background and purpose: Colorectal cancer (CRC) is one of the most common types of cancer. Its incidence has increased in Iran during the last 25 years. Micro-RNAs are used as new approach in cancer researches. The roles of miR-146a have been detected in different tumors. This study aimed at investigating the expression of microRNA146 in tumor tissue of colorectal adenocarcinoma in various stag...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Molecular medicine reports
دوره 6 3 شماره
صفحات -
تاریخ انتشار 2012